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Metal-enhanced bioluminescence presents a great opportunity to achieve ultrasensitive analysis and imaging with low bioluminescent background and enhanced luminescence. We hereby report metal-enhanced bioluminescence based on bioluminescent protein nanocapsules conjugated with gold nanocrystals. Such gold-nanocapsule complexes exhibit near 10-fold enhancement in bioluminescent intensity and are effectively delivered into the cells with outstanding stability. This work offers a class of bioluminescent nanoparticles for imaging and other applications.

Juanjuan Du, Jing Jin, Yang Liu, Jie Li, Talar Tokatlian, Zuhong Lu, Tatiana Segura, Xu-bo Yuan, Xianjin Yang, Yunfeng Lu

Du, J; Jin, J; Liu, Y; Li, J; Tokatlian, T; Lu, Z; Segura, T; Yuan, X-B; Yang, X; Lu, Y